Researchers in the United States have developed a new way of making tough – but soft and wet – biocompatible materials, called “hydrogels”, into complex and intricately patterned shapes.
The process might lead to injectable materials for delivering drugs or cells into the body; scaffolds for regenerating load-bearing tissues; or tough but flexible actuators for future robots, the researchers have claimed.
The process was developed engineers at Massachusetts Institute of Technology (MIT), Duke University, and Columbia University.
Xuanhe Zhao, associate professor of mechanical engineering at MIT, says the new process can produce complex hydrogel structures that are “extremely tough and robust”, and compatible with the encapsulation of cells in the structures. That could make it possible to 3D print complex hydrogel structures – for example, implants to be infused with cells and drugs and then placed in the body.
Hydrogels, defined by water molecules encased in rubbery polymer networks that provide shape and structure, are similar to natural tissues such as cartilage, which is used by the body as a natural shock absorber. The 3D printing process could eventually make it possible to produce tough hydrogel structures artificially for repair or replacement of load-bearing tissues, such as cartilage.
While synthetic hydrogels are commonly weak or brittle, a number of them that are tough and stretchable have been developed over the last decade. However, previous ways of making tough hydrogels have usually involved “harsh chemical environments” that would kill living cells encapsulated in them, said Zhao.
The new materials are benign enough to synthesise together with living cells – such as stem cells – which could then allow high viability of the cells.
In addition, the previous work was not able to produce complex 3D structures with tough hydrogels, Zhao says. The new biocompatible tough hydrogel can be printed into diverse 3D structures such as a hollow cube, hemisphere, pyramid, twisted bundle, multilayer mesh, or physiologically relevant shapes, such as a human nose or ear.
The new method uses a commercially available 3D-printing mechanism, Zhao explained. “The innovation is really about the material – a new ink for 3D printing of biocompatible tough hydrogel,” he said – specifically, a composite of two different biopolymers. “Each [material] individually is very weak and brittle, but once you put them together, it becomes very tough and strong. It’s like steel-reinforced concrete.”
One of the two polymers provides elasticity to the printed material, while the other allows it to dissipate energy under deformation without breaking. A third ingredient, a biocompatible “nanoclay,” makes it possible to fine-tune the viscosity of the material, improving the ability to control its flow through the 3D-printing nozzle.
The material can be made so flexible that a printed shape, such as a pyramid, can be compressed by 99%, and then spring back to its original shape. It can also be stretched to five times its original size. Such resilience is a key feature of natural bodily tissues that need to withstand a variety of forces and impacts.
Such materials might eventually be used to custom-print shapes for the replacement of cartilaginous tissues in ears, noses, or load-bearing joints, said Zhao. Lab tests have already shown that the material is even tougher than natural cartilage.
The next step in the research will be to improve the resolution of the printer, which is currently limited to details about 500 micrometers in size, and to test the printed hydrogel structures in animal models. “We are enhancing the resolution to be able to print more accurate structures for applications,” said Zhao.
In addition to biomedical applications, the same technique could be applied to printing a variety of soft but tough structural materials, he says, such as actuators for soft robotic systems.
